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Empore 96-well Plate

 
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Solid Phase Extraction

Empore™

Standard Well Plate

C18 Sorbent Particles, C8 Sorbent Particles, MPC Sorbent Particles

Life Sciences

Environmental

Basic Research

12

Summary
  • Efficient SPE device with Sorbent particles tightly held together within an inert matrix of polytetrafluoroethylene (PTFE) membrane

  • Sorbent particles tightly enmeshed in PTFE that eliminates particle fines that plug frits

  • Dense particle packing (no void space) & uniform particle distribution results in high separation efficiency

  • Fits standard 96-well plate format

  • Suitable for sample preparation applications for bio-analytical and metabolite analysis protocols

  • Compatible with vacuum manifold and well plate covers

Product Features

Empore™ Well Plates are designed for SPE sample preparation of bioanalytical samples prior to high through-put LC-MS analytical methods. Available with C8, C18, MPC, Empore 96-well plates offer an efficient way to purify variety of bio-analytical samples and allows for robust analytical method development.

 

Empore™ Well Plates are designed for solid phase extraction of analytes from 96 samples. Available with C8, C18, MPC (Mixed Phase Cation - with hydrophobic (C8) and strong cation exchange benzene sulfonic acid) and UR (Universal Resin plate offers both polar, hydrophilic, and nonpolar, hydrophobic, functionalities), Empore™ Well Plates offer an efficient way to purify variety of bio-analytical samples and allows for robust analytical method development. This format is also ideal for examining multiple parameters within a single plate and can reduce the time required for method optimization. The small bed mass of sorbent in Empore™ Well Plates allows for the use of smaller solvent volumes than with traditional packed bed SPE products. Good target analyate recoveries, consistent performance, and clean extracts are the major advantages of the plates.

Empore™ Well Plates provide an effective and efficient means of high throughput solid phase extraction (SPE) by allowing technicians to process 96 samples in a standard 8x12 plate format. This productive format is compatible with standard microtiter liquid handling technologies and injection systems. Parallel sample processing allows 96 samples to be extracted in approximately one hour. Each well of the plate contains the Empore particle-loaded membrane for efficient sample extraction followed by elution in as little as 100μL with mobile phase or mobile phase compatible solutions which can be injected directly onto chromatographic systems. Empore™ Well Plates are available in a standard well, 1.2 mL sample volume size.

The plates are available with the following sorbent phases: URP (Universal Resin), C18, C8, MPC (Mixed Phase Cation).  The C8 and C18 bonded silica sorbents combine good retention capacity with high recoveries.  The MPC (Mixed Phase Cation) bonded silica plates contain both hydrophobic (C8) and strong cation exchange (benzene sulfonic acid) functionalities. This combination of functionalities offers the potential to perform selective extractions of amine containing (basic) analytes or the ability to extract acidic and neutral analytes separately from basic analytes. The Universal Resin (UR) sorbent is a terpolymer based on styrenedivinylbenzene and designed to provide good retention of a wide range of analytes. A single reversed phase method can often be used to isolate and concentrate a variety of acidic, basic and neutral compounds which may reduce method development time.

 
Applications

3MTM EmporeTM (now CDS EmporeTM) C18 Solid Phase Extraction (SPE) disks facilitate rapid and reliable sample preparation and provide excellent analyte recovery for clean chromatograms. This application note demonstrates the performance of such disk in the monitoring of...

This application note shows EmporeTM C18 SPE Disk to effectively extract Polynuclear Aromatic Hydrocarbons (PAHs) from aqueous sample and quantified by GC-FID as the EPA QTM method.

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Empore Products

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Empore Disk

47 and 90 mm disks with C18, C8, SDB-XC, SDB-RPS, Oil & Grease and Activated Carbon

Empore 96-well Plate

96-well plate with 1.2 and 2.5 ml well volume 

Empore Cartridge

1, 3, and 6 mL cartridges with minimum elution volume 

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Empore System

Solid phase extraction instrument

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