This note describes several applications of E3technology™ for proteomic sample preparation, which include two major components, protein cleanup and digestion. The note evaluated different formats of E3technology™, including E3tip, E3filter, and E3plate, and tested a variety of sample types, such as E. coli, yeast, mammalian cells, mouse tissues, and human body fluid. The technology has shown great advantages over existing methods in the context of proteome-wide identification and quantitation. E3technology Empore Proteomics Life Science

This application note describes evaluation of spin columns packed with C18 Empore™ membrane to load and desalt peptides for the purpose of proteomics analysis by LC-MS. Spin columns are shown to exhibit the same desalting ability as popular Empore™ Stage Tips with the advantage of higher loading capacity enabling the ability to process large sample sizes without the need to split sample and risk loss. Spin column Empore Proteomics Life Science

This application note demonstrates sample preparation, detection and quantification of six different microplastics in shellfish using Pyrolysis-GC-MS. Sample preparation involves digestion with potassium hydroxide and cryo-milling. 6000 6150 Pyrolysis Pyroprobe Environmental

A rapid and automated degradation study of rubber under ultraviolet (UV) light in air. 6200 6000 Pyroprobe Pyrolysis Photoprobe UV Degradation

This application note establishes a rapid analysis method for Strontiumin in water in emergency situations. The 1.00 L water sample was acidified and filtered with Empore Strontium solid-phase extraction disk, and then measured Strontium levels on the extraction disk with a low-background α/β counter. The method is simple to operate with stable chemical recovery rates. The lower detection limit of the method is 0.019 Bq/L under typical conditions, and the whole experimental process takes about 2 hours, which is suitable for the rapid determination of Strontiumin in water in emergency situations.

This application note presents RoHS 3 phthalate compliance, and RSDs of phthalates for IEC 62321-8 method using a CDS 6000 Series Pyroprobe. Human Health and Safety Environmental Electronics Pyroprobe 6150 6000

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The analytical performance of the CDS 8500 Series Purge and Trap is demonstrated for the electronic pressure control, used for internal standard delivery, to prepare calibration curves. 8500 P&T Purge & Trap Environmental

The application note developed a new EPA 8270E method by using CDS Empore 8270 disk to maximizes the advantages of the membrane solid-phase extraction technology, which include easy to stack and apply, easy reverse elution, and relatively shorter drying time, etc. The method quantitatively extract a wide range of compounds with most compounds having recovery rates higher than 75%, and the RSDs less than 15%. This method provides a very versatile option for wastewater detection. Empore Environmental

The analytical performance of the CDS 8500 Series Purge and Trap is demonstrated for the sample syringe to prepare calibration curves by performing automated sample dilution. 8500 Purge & Trap Environmental

The Empore™ StageTips product was developed utilizing the Empore™ membrane solid phase extraction (SPE) product to address the need for rapid, offline purification and desalting of peptides in proteomics sample preparation. The structures of SPE reversed phase sorbents is a critical factor to affect the Empore™ StageTips on the types and quantities of peptides extracted. In this application note, 3 types of C18 SPE sorbents and a polymeric reverse phase (Empore™ SDB-XC) have been evaluated on their effects of the corresponding StageTips on total number of protein and peptide Identifications at 4 different sample loading amounts from 10ng to 200ng in proteomics.

This application note introduces a sample-stacking technique to improve sensitivity for low concentration samples. 6000 6200 Pyroprobe Pyrolysis General Interest Environmental